Baza projekata

Detalji projekta<< Pretraživanje


Naziv projekta:
Liječenje neuropatske boli stimulacijom spinalnog ganglija


Voditelj:Vrsta natječaja:
Damir SapunarIstraživački projekti

Rok:Šifra:Akronim:Trajanje:Status:Vrijednost financiranja:
2013-114126NeuroMod15.12.2014 - 14.12.20181.000.000,00 Kn

Znanstvena područja:
Biomedicina i zdravstvo

Znanstvena polja:
Temeljne medicinske znanosti

Ustanova:
Sveučilište u Splitu, Medicinski fakultet

Suradnici:
(univ. bacc.) Adriana Banozic, (univ. bacc.) Antonia Jeličić Kadić, (dr. sc.) Natalija Filipović, (dr. sc.) Sandra Kostić, (prof. dr. sc.) Marija Heffer, (prof. dr. sc.) Livia Puljak, (univ. bacc.) Marta Balog, (univ. bacc.) Senka Blažetić, (dr. sc.) Barbara Viljetić, (univ. bacc.) Vedrana Ivić, (mag. (dipl.) Robert Blažeković, (mag. (dipl.) Ozana Katarina Tot, (dr. sc.) Irena Labak, (prof. dr. sc.) Ivica Grković, (univ. bacc.) Danijel Nejašmić, (prof. dr. sc.) Damir Kovačić, (Doktorand/ PhD student) Tihana Repić, (Doktorand / PhD student) Ivana Vuka,

Ključne riječi:
neuropathic pain, nerve injury, neuromodulation, dorsal root ganglion, ganglionic field stimulation, T-junction, microelectrode arrays

Sažetak:
Neuropathic pain is a debilitating disease of the somatosensory system that has a huge socio-economic impact. Numerous studies, including those from our laboratory have established that the injured dorsal root ganglion (DRG) is the important site for pathophysiologic changes that lead to development of neuropathic pain. Although DRG neurons are critical for the onset of neuropathic pain, there are no relevant clinical treatments for neuropathic pain that target this organ. With this proposal we aim to prove that neurostimulation techniques can be successfully used to manipulate neuronal function at the level of experimentally injured DRGs. In order to test this hypothesis we will construct a fully implantable neuronal stimulator for freely moving rats, devise an implantation technique and define stimulation protocols. This stimulator will be tested in rats with two types of experimentally induced nerve injury; spinal nerve ligation and carrageenan-induced inflammation model. Since this invasive procedure bears substantial risk of inflammation and other adverse neural tissue response, which can induce pain per se, we will also investigate connective tissue fibrosis and neuroinflammatory response in neural tissue of DRG and dorsal horn. The effect of the stimulation on excitability of injured DRG neurons will also be tested in vitro. Our last aim builds on our previous study in which we showed that neuronal injury may disable T-junction filtering and thereby increase the net conduction of afferent traffic. With this proposal we will test whether stimulation can modulate filtering properties of DRG T-junction. This will be addressed using recordings from injured sensory neurons of dorsal root ganglia excised from adult rats, and recently developed methodology that will allow us to electrically visualize action potential propagation in DRG neurons grown over custom microelectrode arrays.